Manual 2ab Assembly Digest

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Add 4uL of Miniprepped DNA to 7uL of either Lefty or Righty MasterMix.

Lefty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BamH1

Righty MasterMix (for 4uL miniprep DNA)

  • 4uL water
  • 1uL of NEB2
  • 1uL XhoI
  • 1uL BglII

Incubate for 1 hour at 37deg C.

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