Result of Bacillus transformation (glycerol stock of competent cells)
strain
antibiotic
added vector
observation
conclusion
IA751
none
none
confluent colonies
cells alive
IA751
Spc50
none
nothing
no contamination
IA751
Spc50
ECE153
15
ok, efficiency 25 colonies/μg of DNA
IA771
none
none
confluent colonies
cells alive
IA771
Cm5
none
nothing
no contamination
IA771
Cm5
ECE166
15
ok, efficiency 5 colonies/μg of DNA
We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells.
Result of starch plates
We had 5mL of iodine solution, wait for 1min and observe.
strain
vector
photo
observation
conclusion
IA751
none
big zones of clearing for each colony
ok
IA771
none
no zone of clearing for each colony
ok
IA751
ECE112/none (29/08)
clearing zones for control, so no trnasformation, it is maybe E.coli, 1 clearing zone out of 3 colonies, it is certainly contamination, but that means that some colonies are transformed
ok
IA751
ECE112 (02/09)
1 zone of clearing out of 4 colonies
some colonies are not transformed and have a resistance to Cm5 (contamination?)
IA751
ECE153 (28/08)
no zone of clearing
5 transformed colonies
IA751
ECE153 (02/09)
2 zone of clearing out of 5 colonies
some colonies are not transformed and have a resistance to Spc50 (contamination?)