IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/07: Difference between revisions
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* Results | * Results | ||
* Lane3 : HyperladderI | |||
* Lane4 : ECE147 | |||
* Lane5 : ECE149 | |||
* Lane6 : ECE150 | |||
* Lane7 : ECE153 | |||
* Lane8 : ECE162 | |||
* Lane9 : HyperladderI | |||
[[Image:photom.gif|300px|center]] | |||
- ECE 147, ECE150, ECE153 :ok! | - ECE 147, ECE150, ECE153 :ok! | ||
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- ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure | - ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure | ||
==Test ECE112 transformation== | ==Test ECE112 transformation== |
Revision as of 04:49, 5 September 2008
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Transformation of B.S. IA771New transformation with the same protocol than 2 days ago. We will try to add less liquid on plates (to avoid growing colonies in liquid)
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Check Vectors
We want to make a final check for ECE147, 149, 150, 153 and 162.
- Double digest + gel
- Results
- Lane3 : HyperladderI
- Lane4 : ECE147
- Lane5 : ECE149
- Lane6 : ECE150
- Lane7 : ECE153
- Lane8 : ECE162
- Lane9 : HyperladderI
- ECE 147, ECE150, ECE153 :ok!
- ECE 149 : 3 bands! not the right vector
- ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure
Test ECE112 transformation
- for the three different strain (1A1, IA771, IA751), make 12 spots
- take one colony with a loop, and put it on a Cm5 plate and on a Cm5 + Spc100 plate (do that for the 12 spots)
- incubate
New stock
- Put ECE171 in 10mL of LB
- Incubate