IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/29: Difference between revisions

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{|class="wikitable" style="text-align:center" border="1"
|-
!  Transformed products !! number of picked colonies from chemical transformation !! number of picked colonies from electrop. transformation (neat) !! number of picked colonies from electrop. transformation (1:10)
|-
| Pupp || 2 || 2 || 0
|-
| Pspac || 2 || 2 || 0
|-
| RBS S || 3 || 0 || 2
|-
| RBS W || 3 || 0 || 2
|-
| agrD || 3 || 2 || 0
|}
- Single colony PCR : 13μL of SDW+cells, 5μL of Master Mix, 1μL of VF and 1μL of VR (and plate each single colony)
- Load a gel (1.3% agarose) : 5μL of PCR products + 1μL of dye (only 1μL of 100b ladder)
{|class="wikitable" style="text-align:center" border="1"
|-
!  Transformed products !! size of the product !! expected size after PCR
|-
| Pupp || 2 || 2 || 0
|-
| Pspac || 2 || 2 || 0
|-
| RBS S || 3 || 0 || 2
|-
| RBS W || 3 || 0 || 2
|-
| agrD || 3 || 2 || 0
|}


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Revision as of 03:25, 1 September 2008

Turing Pattern Formation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Results of transformation with our ligation products

  • Everything grew! Better efficiency with electro> than with chemical protocol

Single colony PCR to check our transformation

Transformed products number of picked colonies from chemical transformation number of picked colonies from electrop. transformation (neat) number of picked colonies from electrop. transformation (1:10)
Pupp 2 2 0
Pspac 2 2 0
RBS S 3 0 2
RBS W 3 0 2
agrD 3 2 0

- Single colony PCR : 13μL of SDW+cells, 5μL of Master Mix, 1μL of VF and 1μL of VR (and plate each single colony)

- Load a gel (1.3% agarose) : 5μL of PCR products + 1μL of dye (only 1μL of 100b ladder)

Transformed products size of the product expected size after PCR
Pupp 2 2 0
Pspac 2 2 0
RBS S 3 0 2
RBS W 3 0 2
agrD 3 2 0


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