IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/28
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Results from transformation of ligation products- nothing on plate, even on control plate
- cells non competent anymore (try with fresh competent cells) - not enough DNA (try with 10μL of DNA) - very short ligation products Transformation of ligation products (new)
- new fresh competent TOP 10 - 5μL of DNA (1.5μL of PUC9) - 2h30 in the incubator |