IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/12: Difference between revisions
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*Lane11 : RBS S4 uncut | *Lane11 : RBS S4 uncut | ||
*Lane12 : supercoiled ladder | *Lane12 : supercoiled ladder | ||
*Result | |||
[[Image:gel21092pcr.gif|400px|center]] | |||
==Check PCR products== | ==Check PCR products== |
Revision as of 02:37, 16 September 2008
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RBS screening (single digest)- Plasmid miniprep 6 colonies of RBS S and 6 colonies of RBS W (without endo wash buffer) - Nanodrop
- Single digest : with EcoRI and XbaI, add SDW and DNA (according to the previous table, 300mg of DNA), 2μL of fast digest buffer, and 1μL of enzyme - Gel1
Check PCR products- Gel (2μL of each product)
- agr B : 2 bands? - agrC : ok - rep : small band but good size - backbones : ok - promoters : ok - RBS : nothing |