IGEM:Harvard/2006/Cyanobacteria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JeffreyLau (talk | contribs) No edit summary |
No edit summary |
||
| Line 17: | Line 17: | ||
[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-1#Plan_for_the_next_few_days | Click here for our current agenda]] | [[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-1#Plan_for_the_next_few_days | Click here for our current agenda]] | ||
==Kai Gene sizes== | |||
:*KaiA size: 855bp | |||
:**903bp with BioBrick ends | |||
:*KaiB: 309bp | |||
:**357bp with BioBrick ends | |||
:*KaiC: 1560bp | |||
==BioBricks Used== | |||
:*<bbpart>BBa_J04450</bbpart> | |||
:**RFP device | |||
:**Insert size: 1069bp | |||
:**[[http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2]] | |||
:***High-copy, Amp<sup>R</sup> | |||
:***Size: 2079bp | |||
:*<bbpart>BBa_J04500</bbpart> | |||
:**Lac promoter + RBS | |||
:**Insert size: 220bp | |||
:**[[http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3]] | |||
:***High-copy, Amp<sup>R</sup>, Kan<sup>R</sup> | |||
:***Insert size: 3189bp | |||
:*[[http://parts.mit.edu/registry/index.php/Part:pSB4A3 pSB4A3]] | |||
:**Low-copy, Amp<sup>R</sup> | |||
:**Insert size: 3339 bp | |||
:*<bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart> | |||
:**GFP device | |||
:**Insert size: 995 bp | |||
==Presentations== | ==Presentations== | ||
Revision as of 11:03, 14 August 2006
<html><style type='text/css'> .tabs {
font-size:80%;
font-weight:none;
width: 100%;
color: #FFFFFF;
background:#FFFFFF url("/images/5/54/DarkgreenTab-bg.gif") repeat-x bottom;
}
.tabs li {
background:url("/images/3/36/DarkgeenTab-left.gif") no-repeat left top;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;
color:#FFFFFF;
padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#CCFF00;
background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:#66FF00;
}
</style></html>
Construct Planning
OMW guys.
Synthesis
Agenda
See image at right for our long-term project outline.
Click here for our current agenda
Kai Gene sizes
- KaiA size: 855bp
- 903bp with BioBrick ends
- KaiB: 309bp
- 357bp with BioBrick ends
- KaiC: 1560bp
- KaiA size: 855bp
BioBricks Used
- <bbpart>BBa_J04450</bbpart>
- RFP device
- Insert size: 1069bp
- [pSB1A2]
- High-copy, AmpR
- Size: 2079bp
- <bbpart>BBa_J04500</bbpart>
- Lac promoter + RBS
- Insert size: 220bp
- [pSB1AK3]
- High-copy, AmpR, KanR
- Insert size: 3189bp
- [pSB4A3]
- Low-copy, AmpR
- Insert size: 3339 bp
- <bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart>
- GFP device
- Insert size: 995 bp
- <bbpart>BBa_J04450</bbpart>
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
- Week 6 progress update, upd. 10:02 7/24 HH
- Week 7 progress update
- Week 8 progress update
- Week 9 progress update
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
List of abbreviations:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
