IGEM:Harvard/2006/Cyanobacteria
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Synthesis
Agenda
See image at right for our long-term project outline.
Monday 6/26
- Figure out optimal growing conditions (complete)
- Regrow cyanobacteria colonies (complete)
- Draft email questions for Professor Golden (complete)
Tuesday 6/27
- PCR crash course (complete)
- Picnic! (complete)
- Check first batch of colonies again (complete)
- Complete more research in preparation for Professor Golden (complete)
- Order primers (complete)
Wednesday 6/28
- Research transformation protocols (mostly complete)
- Start creating presentation for Friday
- Talk to Professor Golden (complete)
- Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
- Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)
Thursday 6/29
- Research Sigma factors (on hold)
- Design and order our first set of primers (for entire KaiABC sequence) (complete)
- Make presentation for Friday (complete)
Friday 6/30
- Present before Fourth of July weekend (cancelled)
Wednesday 7/5
- Research plasmids to ask Prof. Golden (moved from Thursday)
- Also, don't forget to ask her about CO2 and aerating our cultures
- Find out what equipment we would need for measuring bioluminescence
Thursday 7/6
- Make a model of the cyanobacteria
- We missed ice cream at Maxwell-Dworkin
Friday 7/7
- Make a model of the cyanobacteria (complete)
- We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
- PCR PCC7942 (complete-- turned out negative)
- Plate PCC7942 (complete)
- Grow PCC7942 in liquid culture, both with thiosulfate and without (complete)
- TGIF in Bauer Center (complete)
Monday 7/10
- Make frozen stocks of our new grown PCC 7942 (complete)
- Research PCR protocols for cyanobacteria (maybe?) (complete)
- PCR (complete)
- Research DNA synthesis companies (complete)
- Design sequence to be synthesized (incomplete)
- Research homologous recombination design (incomplete)
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
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25 April 2024
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