IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8: Difference between revisions
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| Line 5: | Line 5: | ||
*c3.2H (latch 2, in aptamers) | *c3.2H (latch 2, in aptamers) | ||
*c3.2I (latch 2, out aptamers) | *c3.2I (latch 2, out aptamers) | ||
Final concentration of each oligo in working stocks is 250 nM. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''working stock''' | |||
| align="center" style="background:#f0f0f0;"|'''description''' | |||
| align="center" style="background:#f0f0f0;"|'''pre-working stocks''' (according to [[IGEM:Harvard/2006/Container_Design_3#Working_stocks|chart]]) | |||
| align="center" style="background:#f0f0f0;"|'''water''' | |||
| align="center" style="background:#f0f0f0;"|'''total''' | |||
|- | |||
|c3.2.Fo.core||+latch1<br>+aptamers_in||1 (94 {{ul}}), 2 (28 {{ul}}), 3 (30 {{ul}}), 5 (3 {{ul}}), 6o (5 {{ul}}), 10 (2 {{ul}}), 11 (2 {{ul}}), 13 (0 {{ul}})||36 {{ul}}||200 {{ul}} | |||
|- | |||
|c3.2.Fo.latches||+latch1<br>+aptamers_in||14 (2 {{ul}})||-||- | |||
|- | |||
|c3.2.Go.core||+latch1<br>+aptamers_out||1 (94 {{ul}}), 2 (28 {{ul}}), 3 (30 {{ul}}), 4 (3 {{ul}}), 7o (4 {{ul}}), 10 (2 {{ul}}), 11 (2 {{ul}}), 13 (0 {{ul}})||37 {{ul}}||200 {{ul}} | |||
|- | |||
|c3.2.Go.latches||+latch1<br>+aptamers_out||14 (2 {{ul}})||-||- | |||
|- | |||
|c3.2.Ho.core||+latch2<br>+aptamers_in||1 (94 {{ul}}), 2 (28 {{ul}}), 3 (30 {{ul}}), 5 (3 {{ul}}), 6o (5 {{ul}}), 10 (2 {{ul}}), 12 (2 {{ul}}), 13 (0 {{ul}}), 15 (2 {{ul}})||34 {{ul}}||200 {{ul}} | |||
|- | |||
|c3.2.Ho.latches||+latch2<br>+aptamers_in||16 (4 {{ul}})||-||- | |||
|- | |||
|c3.2.Io.core||+latch2<br>+aptamers_out||1 (94 {{ul}}), 2 (28 {{ul}}), 3 (30 {{ul}}), 4 (3 {{ul}}), 7o (4 {{ul}}), 10 (2 {{ul}}), 12 (2 {{ul}}), 13 (0 {{ul}}), 15 (2 {{ul}})||35 {{ul}}||200 {{ul}} | |||
|- | |||
|c3.2.Io.latches||+latch2<br>+aptamers_out||16 (4 {{ul}})||-||- | |||
|} | |||
[[Design 4]] | [[Design 4]] | ||
*c4.0F (latch 1, in atpamers) | *c4.0F (latch 1, in atpamers) | ||
Revision as of 08:45, 8 August 2006
Folding Rxns w/ New Ligands
- c3.2F (latch 1, in aptamers)
- c3.2G (latch 1, out aptamers)
- c3.2H (latch 2, in aptamers)
- c3.2I (latch 2, out aptamers)
Final concentration of each oligo in working stocks is 250 nM.
| working stock | description | pre-working stocks (according to chart) | water | total |
| c3.2.Fo.core | +latch1 +aptamers_in |
1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6o (5 μL), 10 (2 μL), 11 (2 μL), 13 (0 μL) | 36 μL | 200 μL |
| c3.2.Fo.latches | +latch1 +aptamers_in |
14 (2 μL) | - | - |
| c3.2.Go.core | +latch1 +aptamers_out |
1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7o (4 μL), 10 (2 μL), 11 (2 μL), 13 (0 μL) | 37 μL | 200 μL |
| c3.2.Go.latches | +latch1 +aptamers_out |
14 (2 μL) | - | - |
| c3.2.Ho.core | +latch2 +aptamers_in |
1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6o (5 μL), 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL) | 34 μL | 200 μL |
| c3.2.Ho.latches | +latch2 +aptamers_in |
16 (4 μL) | - | - |
| c3.2.Io.core | +latch2 +aptamers_out |
1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7o (4 μL), 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL) | 35 μL | 200 μL |
| c3.2.Io.latches | +latch2 +aptamers_out |
16 (4 μL) | - | - |
- c4.0F (latch 1, in atpamers)
- c4.0G (latch 1, out aptamers)
- c4.0H (latch 2, in aptamers)
- c4.0I (latch 2, out aptamers)
- c5.0B (latch 2, in aptamers)
- c5.0C (latch 2, out aptamers)
- c6.0A (no aptamers)
- c6.0B (in aptamers)
- c6.0C (out aptamers)
