IGEM:Harvard/2006/Folding buffers: Difference between revisions
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* for a desired final {{mgcl2}} concentration, mix the reagents in a given row | * for a desired final {{mgcl2}} concentration, mix the reagents in a given row | ||
* yields 400 {{ul}} buffer | * yields 400 {{ul}} of 10x buffer | ||
{| {{table}} | {| {{table}} | ||
Revision as of 11:46, 10 August 2006
- for a desired final MgCl2 concentration, mix the reagents in a given row
- yields 400 μL of 10x buffer
| Final [MgCl2] in 10x | Final [MgCl2] in 1x | HEPES (1 M, pH 7.5) | NaCl (5 M) | MgCl2 (1 M) | H2O |
| 100 | 10 | 200 μL | 40 μL | 120 μL | 40 μL |
| 200 | 20 | 200 μL | 40 μL | 80 μL | 80 μL |
| 300 | 30 | 200 μL | 40 μL | 40 μL | 120 μL |
