IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/18: Difference between revisions
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'''Progress''' | '''Progress''' | ||
# Identified allergens in arabidopsis, strawberries,tomatoes, and carrots and created primers for amplifying out these allergens [http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| (see primers and sequences)] | # Identified allergens in arabidopsis, strawberries,tomatoes, and carrots and created primers for amplifying out these allergens [http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| (see primers and sequences)] | ||
# Extracted cDNA from strawberries and tried to PCR out our strawberry allergen (Fra a 1), but were unble ato do so | # Extracted cDNA (rna extraction followed by rt-pcr) from strawberries and tried to PCR out our strawberry allergen (Fra a 1), but were unble ato do so | ||
# Looked to find introns ~ 800 bp to use to create our hairpins since it was difficult to acquire pHannibal vector w/ the pdk intron | # Looked to find introns ~ 800 bp to use to create our hairpins since it was difficult to acquire pHannibal vector w/ the pdk intron | ||
Revision as of 12:04, 18 June 2010
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Week 1 Summary (6/18/2010)Team VectorTeam FlavorTeam AllergyProgress
Future Directions
Team Fence |