IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/29: Difference between revisions

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! T4 DNA ligase buffer
! T4 DNA ligase buffer
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! diH<sub>2</sub>O
! diH<sub>2</sub>O
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Revision as of 08:52, 29 June 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Team Allergy

Digestions

Digestion Reactions
Bet GFP LTP LTP(.5) GFP(.5) Backbone (V0120 w/o a death gene)
DNA 28 28 28 28 28 5
FD Buffer (10x) 4 4 4 4 4 2
diH2O 6 6 6 6 6 11
EcoRI 1 1 1 1 1 1
SpeI 1 1 1 1 1 1


Ligations for full amiRNA and V0120

note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B

For LTP, LTP (.5), Bet For GFP For GFP (.5)

Digestion Reactions
Bet GFP LTP LTP(.5) GFP(.5) Backbone (V0120 w/o a death gene)
DNA 3 5 3 3 4 1
T4 DNA ligase buffer 10 10 10 10 10 align="center"
diH2O 4 2 4 4 3 align="center"

Team Flavor

Results of ligation and transformation of The Big Three (pENTCUP2, NosT, NosT + stop) with B15 backbone into E.coli

A few colonies were found on the pENTCUP2 + Backbone plate, NosT + stop and backbone plate, and b15 backbone control plate. Colonies were picked from the plates and placed in 3ml of LB and agarose in a culture tube. The tubes were placed in the 37°C shaker.

DLT of the Sweethearts - Miraculin and Brazzein (and B21)

Digestion Reactions
Miraculin Brazzein B21
DNA 6 11 7
FD Buffer (10x) 2 2 2
diH2O 10 5 9
EcoRI 1 1 1
SpeI 1 1 1

Placed in 37°C waterbath for 20 minutes.


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