IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/01: Difference between revisions

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(Autocreate 2010/07/01 Entry for IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010)
 
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==Entry title==
==Team Allergy==
* Insert your content here.
 
*'''Ligations for full amiRNA and V0120 (w/o death gene)'''
 
note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B
 
{| border="1"
|+ ''Ligation Reactions''
!  !! Bet!! GFP!! LTP!! LTP(.5)!! GFP(.5)!!
|-
! DNA Insert (after 1:10 dilutions)
| align="center" | 3 || align="center" |  4.5 || align="center" | 3|| align="center" | 4.71|| align="center" | 4.1
|-
! T4 DNA ligase buffer (10x)
| align="center"  | 2 || align="center" | 2 || align="center" | 2|| align="center" | 2|| align="center" |  2
|-
! diH<sub>2</sub>O
| align="center"  | 11.5 || align="center" | 10 || align="center" |  11.5|| align="center"  | 9.79|| align="center"  | 7
|-
! T4 DNA ligase
| align="center"  | 1 || align="center" |  1 || align="center" |  1|| align="center" |  1|| align="center" |  10.4 
|-
! DNA Backbone
| align="center" | 2.5 || align="center" |  2.5|| align="center" | 2.5|| align="center" | 2.5|| align="center" | 2.5
|-
 
|}
 
**Used 50 ng of backbone and 150 ng of insert (ratio of insert to vector ~.14
**Did 1:10 dilutions for the digested inserts




Revision as of 07:35, 1 July 2010

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Team Allergy

  • Ligations for full amiRNA and V0120 (w/o death gene)

note: .5 denotes multistep pcr reaction from yesterday in which .5 uL of part 3 of gfp/ltp were use in one of the giant assemblies of parts 1,2,3 w/ primers A&B

Ligation Reactions
Bet GFP LTP LTP(.5) GFP(.5)
DNA Insert (after 1:10 dilutions) 3 4.5 3 4.71 4.1
T4 DNA ligase buffer (10x) 2 2 2 2 2
diH2O 11.5 10 11.5 9.79 7
T4 DNA ligase 1 1 1 1 10.4
DNA Backbone 2.5 2.5 2.5 2.5 2.5
    • Used 50 ng of backbone and 150 ng of insert (ratio of insert to vector ~.14
    • Did 1:10 dilutions for the digested inserts



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