IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/08: Difference between revisions

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[[Image:PHAN+pKAN_PDK_PCR.jpg‎|240 px]]
[[Image:PHAN+pKAN_PDK_PCR.jpg‎|240 px]]


[[Image:PHAN+pKAN_PDK_PCR2.jpg‎|240 px]
[[Image:PHAN+pKAN_PDK_PCR2.jpg‎|240 px]]


Gel of gradient PCR of the 741bp PDK intron out of pHANNIBAL and pKANNIBAL. Lanes are LtR lex. ordered by (temp, name) with temperatures 50:5:65ºC and names pHAN, pKAN. Improved contrast.
Gel of gradient PCR of the 741bp PDK intron out of pHANNIBAL and pKANNIBAL. Lanes are LtR lex. ordered by (temp, name) with temperatures 50:5:65ºC and names pHAN, pKAN. Improved contrast.

Revision as of 11:25, 8 July 2010

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Entry title

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Team Fence

Team Allergy

  • Minipreps of Allergen Panel
    • Diagnostic Digest

[[Image: |240px]]

  • Gradient PCR of PDK intron

Gel of gradient PCR of the 741bp PDK intron out of pHANNIBAL and pKANNIBAL. Lanes are LtR lex. ordered by (temp, name) with temperatures 50:5:65ºC and names pHAN, pKAN. Improved contrast.

It appears that pHANNIBAL worked at 50, 60ºC but pKANNIBAL didn't.

  • Growing up 5 mL cultures of pHannibal and pKannibal for getting more DNA for pdk pcr


  • Sending out allergen panel plasmids for sequencing



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