IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/14: Difference between revisions
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''8 reactions (Ger S/A; BetS/A; Bet1.2 S/A; LTP S/A)'' | ''8 reactions (Ger S/A; BetS/A; Bet1.2 S/A; LTP S/A)'' | ||
Annealing Temp: 65 C Extension Temp: 30 sec | |||
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|DNA | |DNA | ||
| | |1 uL | ||
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|AB primer mix (1x) | |AB primer mix (1x) | ||
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''Concentrations'': BetS: BetA: GerS: GerA: LTPS: LTPA: Bet1.2S: Bet1.2A: | ''Concentrations'': BetS: 109.1 ng/uL; BetA: 90.5 ng/uL; GerS: 69 ng/uL; GerA: 46 ng/uL; LTPS:36.7 ng/uL; LTPA:5.2 ng/uL; Bet1.2S: 77.4 ng/uL; Bet1.2A:66.2 ng/uL; PME: 64.2 ng/uL; PAL: 62.9 ng/uL; PDK: 35.7 ng/uL; PDK(2): 81.3 ng/uL; PDK(3): 21.5 ng/uL | ||
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Revision as of 06:51, 15 July 2010
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Team FlavorMiniprep of STOP + V0120 partNanodrop O/D's: STOP V0120 1-1: 134.4 ng/μL STOP V0120 1-2: 98.7 ng/μL STOP V0120 2-1: 55.2 ng/μL STOP V0120 2-2: 121.8 ng/μL DTL of StrepII+Mira/Brazz with STOP codon
Digestion of Mira/Brazz+Strep and STOP codon BioBrick
Miraculin + StrepII = 730 bp Brazzein + StrepII = 230 bp STOP + V0120 = 3200 bp
Gel Purification Specs Mira N StrepII E/S: 3.0 ng/μL Mira C StrepII E/S: 2.8 ng/μL Brazz N StrepII E/S: 3.4 ng/μL Brazz C StrepII E/S: 1.3 ng/μL STOP+V0120 BB E/X: 7.1 ng/μL
Team FencePCR of the arabidopsis promoters with the newly extracted DNA and the older DNA. FAIL Arp2 promoter PCR failed. Exp2 promoter PCR failed. Phenol Chloroform DNA Extraction
THE END Team Allergy
Annealing Temp: 65 C Extension Temp: 30 sec
Concentrations: BetS: 109.1 ng/uL; BetA: 90.5 ng/uL; GerS: 69 ng/uL; GerA: 46 ng/uL; LTPS:36.7 ng/uL; LTPA:5.2 ng/uL; Bet1.2S: 77.4 ng/uL; Bet1.2A:66.2 ng/uL; PME: 64.2 ng/uL; PAL: 62.9 ng/uL; PDK: 35.7 ng/uL; PDK(2): 81.3 ng/uL; PDK(3): 21.5 ng/uL |