IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 10: Line 10:
**Redoing Ligations/Transformations of LTPS+PDK
**Redoing Ligations/Transformations of LTPS+PDK
**Sending PDK+V0120 for sequencing
**Sending PDK+V0120 for sequencing
*Looking at sequencing results for our other amiRNA parts
**Bet#2 did not work


*Minipreps of Bet 1S (2); Bet 1.2 S (1); Bet 1.2 A (1)
*Minipreps of Bet 1S (2); Bet 1.2 S (1); Bet 1.2 A (1)
**Repicking colonies for Ger S/A, LTPA, BetA,  
**Repicking colonies for Ger S/A, LTPA, BetA,  


*Looking at PCR results for Allergen Panel from Genomic DNA that we extracted
*Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work)
 
* Ladder, GFPS, GFPA (These first two should not work), LTPS, LTPA, BetS, BetA, Bet2S, Bet2A, GerS, GerA, PME, PAL, PDK
* Ladder, GFPS, GFPA (These first two should not work), LTPS, LTPA, BetS, BetA, Bet2S, Bet2A, GerS, GerA, PME, PAL, PDK


Revision as of 07:39, 15 July 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Allergy

  • Minipreps of PDK+V0120 and GerS+PDK (LTPS+ PDK did not grow)
    • glycerol stocks 500uL of 80% glycerol + 500 uL cells
    • Redoing Ligations/Transformations of LTPS+PDK
    • Sending PDK+V0120 for sequencing
  • Looking at sequencing results for our other amiRNA parts
    • Bet#2 did not work
  • Minipreps of Bet 1S (2); Bet 1.2 S (1); Bet 1.2 A (1)
    • Repicking colonies for Ger S/A, LTPA, BetA,
  • Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work)
  • Ladder, GFPS, GFPA (These first two should not work), LTPS, LTPA, BetS, BetA, Bet2S, Bet2A, GerS, GerA, PME, PAL, PDK

[[Image: |240px|allergen panel from arabidopsis DNA extraction]]

  • Digest and Ligate into V0120 and Transform
    • Tmrw: grow up cultures for miniprepping and sending out for sequencing

Team Fence

Team Flavor

Team Vector


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/07/15&oldid=433084"