IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/15: Difference between revisions
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*Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work) | *Looking at PCR results for Allergen Panel from Genomic DNA that we extracted (did not work) | ||
Ladder, GFPS, GFPA (These first two should not work), LTPS, LTPA, BetS, BetA, Bet2S, Bet2A, GerS, GerA, PME, PAL, PDK | |||
[[Image: |240px|allergen panel from arabidopsis DNA extraction]] | [[Image: |240px|allergen panel from arabidopsis DNA extraction]] |
Revision as of 11:57, 15 July 2010
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Team Allergy
Ladder, GFPS, GFPA (These first two should not work), LTPS, LTPA, BetS, BetA, Bet2S, Bet2A, GerS, GerA, PME, PAL, PDK [[Image: |240px|allergen panel from arabidopsis DNA extraction]] Team FenceTeam FlavorMiraculin/Brazzein YFP N/C and NosT + Stop in V24 DUET vector
ODs Miraculin C1: 168.8 ng/μL Miraculin C2: 67.2 ng/μL Miraculin N1: 183.0 ng/μL Miraculin N2: 173.5 ng/μL Brazzein N1: 54.8 ng/μL Brazzein N2: 79.0 ng/μL Brazzein C1: 161.4 ng/μL Brazzein C2: 125.0 ng/μL
Expected Lengths Miraculin + YFP + NOSt + STOP = 700 + 1500 + 250 = 2450 bp Brazzein + YFP + NOSt + STOP = 300 + 1500 + 250 = 2050 bp V24 = 5200 bp Team Vector |