IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/26: Difference between revisions
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PFX polymerase PCR | PFX polymerase PCR | ||
1μL 10mM MgSO4 | *1μL 10mM MgSO4 | ||
2μL 10X pfx amplification buffer | *2μL 10X pfx amplification buffer | ||
2μL 10X PCRx enhancer solution | *2μL 10X PCRx enhancer solution | ||
1μL F primer | *1μL F primer | ||
1μL R primer | *1μL R primer | ||
0.5μL pfx polymerase | *0.5μL pfx polymerase | ||
2μL DNTP | *2μL DNTP | ||
9.5μL total | 9.5μL total | ||
DNA: 21ng/μL | DNA: 21ng/μL | ||
50 to 200μL DNA per reaction | *50 to 200μL DNA per reaction | ||
5 reactions | *5 reactions | ||
4μL per reaction = 20μL sample total (MM) | *4μL per reaction = 20μL sample total (MM) | ||
9.5μL + 4μL = 13.5μL | 9.5μL + 4μL = 13.5μL | ||
| Line 29: | Line 29: | ||
MM | MM | ||
5μL 10nM MgSO4 | *5μL 10nM MgSO4 | ||
10μL 10X pfx amplification buffer | *10μL 10X pfx amplification buffer | ||
10μL 10X PCRx enhancer solution | *10μL 10X PCRx enhancer solution | ||
5μL F primer | *5μL F primer | ||
5μL R primer | *5μL R primer | ||
2.5μL pfx polymerase | *2.5μL pfx polymerase | ||
10μL DNTP | *10μL DNTP | ||
*20μL genomic DNA | |||
*32.5μL H20 | |||
total volume:100μL | |||
==Team Allergy== | ==Team Allergy== | ||
Revision as of 11:02, 26 July 2010
 iGEM iGarden
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Team FencePFX polymerase PCR
9.5μL total DNA: 21ng/μL
9.5μL + 4μL = 13.5μL therefore 6.5μL H20 per reaction MM
total volume:100μL Team Allergy
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