IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/04

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(Entry title)
(Team Allergy)
Line 14: Line 14:
**Realized that we hadn't gel purified our inserts  
**Realized that we hadn't gel purified our inserts  
**Gel purification of inserts (entire ihpRNA parts)
**Gel purification of inserts (entire ihpRNA parts)
-
[[Image: |240px|Gel 1]]  [[Image: |240px|Gel 2]]   
+
[[Image: Gelpurinsert.jpg|240px|Gel 1]]  [[Image: |240px|Gel 2]]   
Gel 1:  Ladder, 9, 11c1, 11c2, ladder, 25c1, 28c1, 28c2, 36c1, 36c2, ladder
Gel 1:  Ladder, 9, 11c1, 11c2, ladder, 25c1, 28c1, 28c2, 36c1, 36c2, ladder
Gel 2: Ladder, 37c1, 37 c2, 38c1, 38c2, ladder
Gel 2: Ladder, 37c1, 37 c2, 38c1, 38c2, ladder

Revision as of 16:18, 4 August 2010

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Team Allergy

  • amiRNA
  • V9/V10
    • Diagnostic digest of our gel :

[[Image: |240 px]]

(Should see ~ 7kb band and an 840 band)

    • Realized that we hadn't gel purified our inserts
    • Gel purification of inserts (entire ihpRNA parts)

Gel 1 [[Image: |240px|Gel 2]] Gel 1: Ladder, 9, 11c1, 11c2, ladder, 25c1, 28c1, 28c2, 36c1, 36c2, ladder Gel 2: Ladder, 37c1, 37 c2, 38c1, 38c2, ladder




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