IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/06: Difference between revisions
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*no colonies on plates from transformations done yesterday after 16 hours (we let these plates grow for longer) | *no colonies on plates from transformations done yesterday after 16 hours (we let these plates grow for longer) | ||
*redoing ligations of ihpRNA inserts in V9/V10 | *redoing ligations of ihpRNA inserts in V9/V10 | ||
**Redigested and gel purified some backbone | **Redigested and gel purified some backbone | ||
[[Image: |240px|V9 and V10 backbone]] | |||
''Concentrations:'' V9 (5 ng/uL); V10 (6 ng/uL) | |||
**12 ligations each for V9 and V10 | |||
==Team Flavor== | ==Team Flavor== |
Revision as of 10:21, 6 August 2010
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Team AllergyamiRNA
ihpRNA
[[Image: |240px|V9 and V10 backbone]] Concentrations: V9 (5 ng/uL); V10 (6 ng/uL)
Team FlavorConfirmation Digest of J45004
Backbone: 3.2 kb; Insert: 1.1 kb AnalysisBoth the backbone and the insert in lane 1 look like the correct length (3.2 and 1.1 kb respectively). Lane two consists of a backbone of the correct length, but the insert appears to be ~1.5 kb, too long for the J45004 BioBrick part. Lane 3 appears undigested; we are uncertain as to why.
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