IGEM:Harvard/2010/Brainstorming/food: Difference between revisions
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Dual selection system (aka killswitch) ideas:<br> | ==Strains, Plasmids, and Trasformation== | ||
*References | |||
*protocols | |||
==Markers and Kill Switches== | |||
*Dual selection system (aka killswitch) ideas:<br> | |||
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not<br> | - use selectable markers such as URA3 to control whether an integration plasmid is replicated or not<br> | ||
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)<br> | -- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)<br> | ||
-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br> | -- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br> | ||
==Downstream effectors== | |||
*allergens | |||
*flavors | |||
*colors | |||
*fluorescent proteins | |||
Revision as of 14:52, 28 April 2010
Strains, Plasmids, and Trasformation
- References
- protocols
Markers and Kill Switches
- Dual selection system (aka killswitch) ideas:
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
Downstream effectors
- allergens
- flavors
- colors
- fluorescent proteins
