IGEM:IMPERIAL/2006/Results/ABS490 Calibration

Aim

The aim of this experiment was to make a calibration curve to be able to convert Abs490, which is measured by the plate reader, into OD600.

Method

• A dilution series of different OD600s was created using the in-lab spectrophotometer and overnight culture of cells.
• These dilutions were also assessed via the spectrophotometer to make sure they were near their targetted OD.
• 3 x 200μL of each dilution was pipetted into a 96 well plate and then Abs490 was read by the Victor3 plate-reader
• The cells and the dilutions were kept on ice to prevent any increase in OD

Results

• Data File:
  • Abs490 -> OD600 Calibration Data