IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(3 intermediate revisions by one other user not shown)
Line 22: Line 22:
</div>
</div>
<br style="clear:both">
<br style="clear:both">
==Ligation strategy==


<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
<u><font size="2">'''Ligating the Polycystronic Predator Construct:'''</font></u>
Line 66: Line 68:
!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
!style="background:#D9F2ED"|<center><bbpart>J37035</bbpart>=<br>(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)</center>|| || || || !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png|30px]][[Image:B0034 logo.png|30px]][[Image:C0062 logo.png|30px]][[Image:B0014 logo.png|50px]][[Image:R0062 logo.png|30px]][[Image:J37023 logo.png|30px]][[Image:J37024 logo.png|70px]]</center>
|}
|}
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].


==Quality control==
==Quality control==
Line 75: Line 79:
| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || N/A
| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || N/A
|-
|-
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
Sequence: TAT TGT CTC ATG AGC GG<br>
Seconday Structure: None
|-
|-
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] <br> Note: This part was sequenced as a part of J37025 || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
|-
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A
|-
|-
| <center><bbpart>J37025</bbpart></center> || [[Image:J37025 logo.png]] || No || N/A || N/A
| <center><bbpart>J37025</bbpart></center> || [[Image:J37025 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
|-
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || No || N/A
|-
|-
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || No || N/A
|-
|-
| <center><bbpart>J37035</bbpart></center> || [[Image:J37035 logo.png]] || No || N/A || N/A
| <center><bbpart>J37035</bbpart></center> || [[Image:J37035 logo.png]] || No || N/A || N/A
Line 97: Line 99:


*the final assembly was not successful
*the final assembly was not successful
*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information]
*Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).
<html>
<!-- Start of StatCounter Code -->
<script type="text/javascript" language="javascript">
var sc_project=1999441;
var sc_invisible=1;
var sc_partition=18;
var sc_security="18996820";
</script>
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img  src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript>
<!-- End of StatCounter Code -->
</html>

Latest revision as of 09:43, 30 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligation strategy

Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37018</bbpart>
 No N/A N/A
<bbpart>J37019</bbpart>
 Yes Yes Primers used
<bbpart>J37023</bbpart>
 Yes Yes
Note: This part was sequenced as a part of J37025		 Primers used
<bbpart>J37024</bbpart>
 No N/A N/A
<bbpart>J37025</bbpart>
 Yes Yes Primers used
<bbpart>J37031</bbpart>
 Yes No N/A
<bbpart>J37033</bbpart>
 Yes No N/A
<bbpart>J37035</bbpart>
 No N/A N/A
<bbpart>J37036</bbpart>
 No N/A N/A

WetLab work entries

  • the final assembly was not successful
  • The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
  • Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).

<html> <!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=1999441; var sc_invisible=1; var sc_partition=18; var sc_security="18996820"; </script>

<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct/Implementation&oldid=83904"