IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct

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Super Parts	Predator Construct
Actual Part
Sub Parts	<bbpart>R0062</bbpart>	<bbpart>B0034</bbpart>	<bbpart>C0062</bbpart>	<bbpart>I13504</bbpart>

Motivation

• The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
• The Aiia gene has been replaced with GFP to allow direct measurement of protein production
• From this data we can create a transfer function where AHL is the input and GFP the output
• Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
• The part will be used to form the sensing part of the final predator construct

Parts Interface

INPUTS OUTPUTS AHL AHL-lactonase & fluctuating [AHL]	Predator construct at the device level.
INPUTS OUTPUTS AHL AHL-lactonase	Predator sensing test construct.
INPUTS OUTPUTS AHL GFP	Predator sensing test construct.

Expected behavior and performances

System level specifications

Properties	Specifications
Input Range(s)	[0.01nm,10000nm]
Output Range(s)	?
Transfer function(s)
Robustness	Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability	Variability at the device population level (day to day, device to device) should be as low as possible.