IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

Super Parts	Molecular Prey-Predator Oscillator
Actual Part
Sub Parts	Test Sensing Predator Construct	Test Killing Predator Construct

Ligation strategy

Ligating the Polycystronic Predator Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4
<bbpart>J37023</bbpart> (<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
<bbpart>J37025</bbpart>= (<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37036</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4	Stage 5
<bbpart>J37023</bbpart> (<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>= (<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>= (<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>= (<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Part	Built	Sequenced	Primers Used for Sequencing
<bbpart>J37018</bbpart>	No	N/A	N/A
<bbpart>J37019</bbpart>	Yes	Yes	Forward pSB1(AK3/A3/A2) Sequence: TAT TGT CTC ATG AGC GG Seconday Structure: None
<bbpart>J37023</bbpart>	Yes	?	?
<bbpart>J37024</bbpart>	No	N/A	N/A
<bbpart>J37025</bbpart>	No	N/A	N/A
<bbpart>J37031</bbpart>	?	?	?
<bbpart>J37033</bbpart>	Yes	?	?
<bbpart>J37035</bbpart>	No	N/A	N/A
<bbpart>J37036</bbpart>	No	N/A	N/A