IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct: Difference between revisions
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*The resulting transfer curve will be used to extract parameters Vmax and Kd to characterize the part and for use in our modelling. | *The resulting transfer curve will be used to extract parameters Vmax and Kd to characterize the part and for use in our modelling. | ||
*This part will form the receiver of the final predator construct | *This part will form the receiver of the final predator construct | ||
==Parts Interface== | |||
[[Image:IGEM IMPERIAL PreadatorConstructSystemIcon.png|thumbnail|right|400px|Predator construct at the system level.]] | |||
[[Image:PredatorAtDeviceLevel.png|thumbnail|right|400px|Predator construct at the device level.]] |
Revision as of 01:25, 22 October 2006
Super Parts | Predator Construct | |||
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Actual Part | ||||
Sub Parts |
Motivation
- To mimic Lotka-Volterra, the predator construct is split into a 'sensing part' and 'killing part'.
- The sensing part of the predator produces AHL lactonase as a function of the AHL produced by the prey
- In this part the AiiA gene has been replaced with the reporter gene GFP to allow the measurement of the production of GFP as a function of AHL.
- The resulting transfer curve will be used to extract parameters Vmax and Kd to characterize the part and for use in our modelling.
- This part will form the receiver of the final predator construct