IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct
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Motivation
- To mimic Lotka-Volterra, the predator construct is split into a 'sensing part' and 'killing part'.
- The sensing part of the predator produces AHL lactonase as a function of the AHL produced by the prey
- In this part the AiiA gene has been replaced with the reporter gene GFP to allow the measurement of the production of GFP as a function of AHL.
- The resulting transfer curve will be used to extract parameters Vmax and Kd to characterize the part and for use in our modelling.
- This part will form the receiver of the final predator construct
Parts Interface
