IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation: Difference between revisions

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==Quality control==
==Quality control==
*define the quality control used at each step
*These parts were sequenced to determine the correct sequence
*define possible enzymes to be used and their outcomes
*define design of primers to be used in sequencing


__NOTOC__
__NOTOC__
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!height="25pt" width="80pt"| Part !! Built !! Sequencing Results !! Primers Used for Sequencing
!height="25pt" width="80pt"| Part !! Built !! Sequencing Results !! Primers Used for Sequencing
|-
|-
|Put part logo and name here || has it been built? || sequencing analysis and link to sequence results || primers used
|Put part logo and name here || Yes || sequencing analysis and link to sequence results || PSBA2 primers
|-
|-
|Put part logo and name here || has it been built? || sequencing analysis and link to sequence results || primers used
|Put part logo and name here || Yes || sequencing analysis and link to sequence results || PSBA2 primers
|-
|-
|}
|}

Revision as of 08:36, 28 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Predator Test Construct

These two parts are very similar and have the same inputs and outputs, therefore they can be analysed in exactly the same way



This construct is used to characterise the pLuxR transfer function, and noteably its effect on YFP expression, which would be equivalent to AiiA expression in the full system with one promoter.




This construct is used to characterise the pLuxR transfer function, and noteably its effect on YFP expression, which would be equivalent to AiiA expression in the full system with two promoters.

The input is HSL and the outputs are LuxR and GFP. GFP will be mesured

Quality control

  • These parts were sequenced to determine the correct sequence


Part Built Sequencing Results Primers Used for Sequencing
Put part logo and name here Yes sequencing analysis and link to sequence results PSBA2 primers
Put part logo and name here Yes sequencing analysis and link to sequence results PSBA2 primers
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