IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation: Difference between revisions

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==Ligation strategy==
<u><font size="2">'''Ligating the Polycystronic Predator 'Sensing' Test Construct:'''</font></u>
<u><font size="2">'''Ligating the Polycystronic Predator 'Sensing' Test Construct:'''</font></u>
__NOTOC__
__NOTOC__
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!style="background:#D9F2ED"|<center><bbpart>J37020</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)</center>|| || || !!style="background:#CCEBAD" | [[Image:J37020 logo.png]]
!style="background:#D9F2ED"|<center><bbpart>J37020</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)</center>|| || || !!style="background:#CCEBAD" | [[Image:J37020 logo.png]]
|}
|}
*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].


==Quality control==
==Quality control==
*These parts were sequenced to determine the correct sequence


__NOTOC__
__NOTOC__
{| border="1" cellpadding="0" width="100%"
{| border="1" cellpadding="0" width="100%"
|-
|-
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequencing Results !! Primers Used for Sequencing
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
|-
|-
| <bbpart>BBa_J37016</bbpart> ||jkhkh|| Yes || sequencing analysis and link to sequence results || PSBA2 primers
| <center><bbpart>J37016</bbpart></center> || [[Image:J37016 logo.png]] || Yes || [http://openwetware.org/images/4/4d/J37016_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
|-
|-
| <bbpart>BBa_J37020</bbpart> ||kljkljkl|| Yes || sequencing analysis and link to sequence results || PSBA2 primers
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
|-
|-
| <center><bbpart>J37020</bbpart></center> || [[Image:J37020 logo.png]] || No || N/A || N/A
|-
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || [http://openwetware.org/images/a/ac/J37020sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers]
|-
| <center><bbpart>J37032</bbpart></center> || [[Image:J37032 logo.png]] || Yes || No || N/A
|-
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || No || N/A
|}
|}
==WetLab work entries==
*The polycystronic predator sensing part (J37016) has been successfully built, and tested.
*The monocystronic predator sensing part (J37020) was not built.

Latest revision as of 07:47, 30 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligation strategy

Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37016</bbpart>
 Yes Yes Primers
<bbpart>J37019</bbpart>
 Yes Yes Primers
<bbpart>J37020</bbpart>
 No N/A N/A
<bbpart>J37031</bbpart>
 Yes Yes Primers
<bbpart>J37032</bbpart>
 Yes No N/A
<bbpart>J37033</bbpart>
 Yes No N/A

WetLab work entries

  • The polycystronic predator sensing part (J37016) has been successfully built, and tested.
  • The monocystronic predator sensing part (J37020) was not built.
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