IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.1: Difference between revisions
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===<div style="color:darkblue; font-size: 140%;">GFP with variable water volumes</div>=== | ===<div style="color:darkblue; font-size: 140%;">GFP with variable water volumes</div>=== | ||
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Revision as of 04:55, 6 September 2007
Results Summary
GFP with variable water volumes
This purpose of this experiment was to investigate whether the same number of moles of GFP, in different water volumes gave a different fluorescence reading or not. Depending on the results, we would see whether we needed or not to maintain constant volumes when making GFP dilutions for our calibration curve.
We therefore added 4 different volumes of water in 4 wells and in each well we added the same amount of GFP (from the same stock soluton). Two wells were also used to contain just GFP(+ve) and just water(-ve) as a controls. The results are shown below:
On the graph on the right you can see the fluorescence reading of the pLux assembly 7 hours after it has been induced with AHL. The readings of pLux are comparable to the diluted GFP solution and leads us to conclude that GFP has been expressed. Thus the contruct works as expected in vivo. Further tests will need to be conducted to reveal its sensitivity to AHL.
