IGEM:IMPERIAL/2007/Projects/Experimental Design
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We have identified three phases of experiments for each of the project:
- Phase 1 - Intial testing of the promoters and test constructs in vitro
- Phase 2 - Testing/Validation of the test constructs
- Phase 3 - Testing/Validation of final constructs
In addition any overlap between the experiments of the projects has been identified and considered when making the experimental design.
Status
- Several Problems have been identified, we have begun to address them. Link here to problems.
- We are currently trying to improve our methodology to get better results. Link to improving our Methodology
- Planned experiments for future
Time table
General timetable
| Week 8 | Week 9 | Week 10 | |
| Monday | - | Refine the wiki | Planning the week |
| Tuesday | Prepare GFP solution-calibration Construct Calibration Curve Refine the wiki |
Testing to reduce evaporation | |
| Wednesday | Test Home made cell extract Test varying levels of cell extract |
PROBLEM-Wrong GFP!!!! | Purify GFP DsRED arrives?? 24hr experiment for mineral oil method |
| Thursday | Purify GFP Prepare DsRED constructs | ||
| Friday | Prepare GFP solution-calibration Construct Calibration Curve |
Purify GFP + Test Concentration Prepare DsRED constructs |
CBD
| Week 8 | Week 9 | Week 10 | |
| Monday | - | ||
| Tuesday | Phase 1 - test pT7 | ||
| Wednesday | 24 Hour Exp Room Temp | ||
| Thursday | Test 20 and 37 °C, Test GFP degradation at 20 and 37 °C |
Define the thresholds of temperature | |
| Friday | Second day testing 20 and 37 °C |
ID
| Week 8 | Week 9 | Week 10 | |
| Monday | - | Purify LuxR | |
| Tuesday | test pLux in-vitro - 1nM AHL | Refine AHL concentrations range 25°C Purify LuxR if plasmid arrives |
Purified LuxR ready today |
| Wednesday | Purify LuxR if plasmid arrives |
Test Purified LuxR with construct 2 | |
| Thursday | Clone in plasmid | ||
| Friday | Test construct with 5 AHL concentrations - 25 °C | Begin purification of LuxR |
