IGEM:IMPERIAL/2007/Projects/Experimental Design/Future Experiments

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Future Tests

(CE:Number of Cell extracts needed)

  1. Calibration curve GFP (CE:10)
  2. Calibration curve dsRED (CE:10)
  3. ID - Final Testing of AHL for dsRED (CE:20)
  4. CBD - Steep Gradient dsRED- ( Ask Alex about final app)
    • Slow Gradient dsRED
    • Final Test Temp range for DsREd(CE:20)

Total Cell Extracts Reactions Required For Project

  • Week 10: 20
  • Future testing: 60
  • In Stock: 72 Reactions

Dependencies

  1. DsRed construct ( Tuesday)
  2. Maxi Preps:
    • pLux Construct 2 - Wait for intial testing
    • Negative Control - Constuct rbs:GFP:Terminator, No promoter
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