IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results2: Difference between revisions
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== | ==Aims== | ||
The experiemtnt was carried out on the [[IGEM:IMPERIAL/2007/Notebook/2007-9-11 | 11-09-2007]] | The experiemtnt was carried out on the [[IGEM:IMPERIAL/2007/Notebook/2007-9-11 | 11-09-2007]] | ||
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==Results== | |||
A summary and graph of the results are shown below. The results show us that using 20ul and above of mineral or paraffin oil will prevent evaporation over a 17 hour period. Using 90ul seemed to be more effective because although 20ul did clearly stop alot of evaporation, some was still present. However, two problems were found when measuring: | A summary and graph of the results are shown below. The results show us that using 20ul and above of mineral or paraffin oil will prevent evaporation over a 17 hour period. Using 90ul seemed to be more effective because although 20ul did clearly stop alot of evaporation, some was still present. However, two problems were found when measuring: | ||
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Revision as of 01:21, 13 September 2007
Aims
The experiemtnt was carried out on the 11-09-2007
Results
A summary and graph of the results are shown below. The results show us that using 20ul and above of mineral or paraffin oil will prevent evaporation over a 17 hour period. Using 90ul seemed to be more effective because although 20ul did clearly stop alot of evaporation, some was still present. However, two problems were found when measuring:
- Measuring oil accuratly is difficult because of the capillary action of the gilson pipette
- Measuring the samples out of the wells because of the difficultly in pipetting small volumes from the wells.
From the results we have decided to use above 20ul in our experiments this is because we feel that although evaporation is minimal we need to try to prevent any evaporation. To determine the exact amount we will look at the results from the test of fluorescence with oil.
In terms of methodology we have noted that pipetting mineral oil slowly and with lower volumes improves upon problem 1 above.
Data
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