IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase2: Difference between revisions

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*Positive Control: In vitro system with a construct gaurenteed to work
*Positive Control: In vitro system with a construct gaurenteed to work
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Revision as of 06:51, 15 August 2007



1. Common GFP Experiments



1.1 GFP Calibration Curve


Status
Planned for 20/8/07 INCOMPLETE

Test to determine the relationship between fluorescence and in vitro concentration of GFP. To test this purified samples of known [GFP] are added to in vitro chassis and the fluorescence measured. From this a calibration curve of [GFP] vs Fluorescence can be made. This can be used for data analysis to convert fluorescence into a [GFP].


Protocol
Results

Aims:

  • To determine [GFP] vs Fluorescence


[+] Constant Conditions

[+] Variables

[+] Sampling

[+] Controls

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1.2 Degradation Time of GFP


Status
Planned for 20/8/07 INCOMPLETE

Test the half life of GFP protein in an in vitro chassis. To test this a purified sample of known [GFP] are added to an in vitro chassis, then fluorescence will be measured at regular time intervals. The fluorescence will be converted into GFP molecules using the calibration curve. This will give; degradation of GFP as a function of time, from this the half life of GFP can be obtained. In addition, temperature may affect the half life of GFP and so the half life will be measured for an appropriate temperature range.

Protocol
Results

Aims:

  • To determine the half life of GFP for a range of temperatures


[+] Constant Conditions:

[+] Variables:

[+] Sampling:

[+] Controls:

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2. Project Specific Experiments

[Cell by Date]

[Infector Detector]