IGEM:IMPERIAL/2007/Projects/In-Veso/Notes: Difference between revisions
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(New page: =In-Veso Gene Expression: Notes= __NOTOC__ <div class="tabs-blue"> <ul> <li>Introduction</li> <li>[[IGEM:IMPERIAL/2007/Projects/In-Veso/Specificati...) |
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==Timeline== | |||
===Week 4=== | |||
'''Mon''': Order reagents | |||
'''Tue''': - | |||
'''Wed''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract | |||
'''Thur''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract | |||
'''Fri''': Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract | |||
===Week 5=== | |||
'''Mon''': Test if S30 or S12 is better for the respective strains of E.coli | |||
'''Tue''': Carry out temperature and pH tests on in vitro and in veso extracts | |||
'''Wed''': Carry out temperature and pH tests on in vitro and in veso extracts | |||
'''Thur''': Carry out temperature and pH tests on in vitro and in veso extracts | |||
'''Fri''': Carry out temperature and pH tests on in vitro and in veso extracts | |||
Revision as of 15:25, 29 July 2007
In-Veso Gene Expression: Notes
Timeline
Week 4
Mon: Order reagents Tue: - Wed: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract Thur: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract Fri: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Week 5
Mon: Test if S30 or S12 is better for the respective strains of E.coli Tue: Carry out temperature and pH tests on in vitro and in veso extracts Wed: Carry out temperature and pH tests on in vitro and in veso extracts Thur: Carry out temperature and pH tests on in vitro and in veso extracts Fri: Carry out temperature and pH tests on in vitro and in veso extracts
