IGEM:IMPERIAL/2007/Projects/In-Veso/Modelling

In-Veso Gene Expression: Modelling

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Shear Force in Emulsification Process

Inputs

• RPM of mixer during mixing process
• Surface area of mixing container
• Possibly others, such as temperature, phase composition, pressure

Outputs

• Shear force experienced by emulsion

Relating Shear and Duration to Vesicle Population and Volume

Inputs

• Duration of mixing process
• Shear force experienced by emulsion

Outputs

• Average size of vesicles
• Standard deviation of vesicle size
• Density of vesicle population

Relating Centrifugation Speed and Duration to Interface Transfer

Inputs

• Centrifuge speed
• Duration of centrifugation
• Density of vesicles in emulsion

Outputs

• Density of vesicles in solution