IGEM:IMPERIAL/2007/Projects/In-Veso/Notes: Difference between revisions
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<br>'''Thur''': Carry out temperature and pH tests on in vitro and in veso extracts | <br>'''Thur''': Carry out temperature and pH tests on in vitro and in veso extracts | ||
<br>'''Fri''': Carry out temperature and pH tests on in vitro and in veso extracts | <br>'''Fri''': Carry out temperature and pH tests on in vitro and in veso extracts | ||
===Proposal for Lab=== | |||
We will first work on making gene constructs and cloning them in E.coli. Restriction digestion, ligation and transformation will be used. Agarose gell electrophoresis will then be used to test for products formed. Cell extracts will be bought from Molecular biology suppliers. Miniprep will be performed to extract plasmids from the cells. | |||
Revision as of 02:51, 31 July 2007
In-Veso Gene Expression: Notes
Timeline
Week 4
Mon: Order reagents
Tue: -
Wed: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Thur: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Fri: Make gene construct of T7 and pLux promoters, Make S30 extract, Make S12 extract
Week 5
Mon: Test if S30 or S12 is better for the respective strains of E.coli
Tue: Carry out temperature and pH tests on in vitro and in veso extracts
Wed: Carry out temperature and pH tests on in vitro and in veso extracts
Thur: Carry out temperature and pH tests on in vitro and in veso extracts
Fri: Carry out temperature and pH tests on in vitro and in veso extracts
Proposal for Lab
We will first work on making gene constructs and cloning them in E.coli. Restriction digestion, ligation and transformation will be used. Agarose gell electrophoresis will then be used to test for products formed. Cell extracts will be bought from Molecular biology suppliers. Miniprep will be performed to extract plasmids from the cells.
