IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications: Difference between revisions
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=== Protocol === | === Protocol === | ||
[[Image:S30S12.gif]] | |||
Above are two choices for making cell extract from E.coli. One of them is S30, and the other is the S12 method, which is a more rapid and cost-effective preparation. The cell extracts can also be obtained from a manufacturer. | |||
<br>Below is a protocol for obtaining the cell extract S30. | |||
=== Rate of PoPs === | === Rate of PoPs === | ||
=== Comparison with the efficiency of other chassis === | === Comparison with the efficiency of other chassis === |
Revision as of 08:30, 26 July 2007
In-Veso Gene Expression: Specifications
Characterization
Resources
IC Specialists
Literature
Wetlab
Characterisation Chart
PoPS Effect | Lifespan | Protocol | Membrane Traffic | Waste | |
Temperature | |||||
Media | |||||
pH | |||||
Piping | |||||
Centrifuge |
Temperature
Range from 0-40oC
Media
These are the different media the technique should be tested in, in order of priority:
- Water
- Agar Gel
- β-Galactosidase
- Polyacrylamide
- Paper
- E. coli Biofilm
Tom 10:56, 26 July 2007 (EDT) Just a note to take into account Osmolarity of the media, which I'm sure you've thought of already! Also, B-Galactosidase is an enzyme not a type of media?
pH
Range from 6.0 to 8.0
Piping
How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?
This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.
Centrifuge
How fast can the vesicles be centrifuged without destruction? Testing range...?
Protocol
Above are two choices for making cell extract from E.coli. One of them is S30, and the other is the S12 method, which is a more rapid and cost-effective preparation. The cell extracts can also be obtained from a manufacturer.
Below is a protocol for obtaining the cell extract S30.
Rate of PoPs
Comparison with the efficiency of other chassis
Limitations of type of proteins made in the vesicles
Life span
Membrane Permeability
Physical Conditions
temperature, pH, media, centrifugation