IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications: Difference between revisions
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List of parts from the registry we would like to test in-veso. | List of parts from the registry we would like to test in-veso. | ||
=== Rate of PoPs === | === Rate of PoPs === |
Revision as of 08:54, 26 July 2007
In-Veso Gene Expression: Specifications
Characterization
Resources
IC Specialists
Literature
Wetlab
Characterisation Chart
Lifespan | Rate of Protein Synthesis |
PoPS Effect | Membrane Traffic | Waste | |
Temperature | |||||
Media | |||||
Piping | |||||
Parts |
Temperature
Range from 4-37oC with priorities at:
- 25oC
- 4oC
- 6-20oC
- 30-37oC
Media
These are the different media the technique should be tested in, in order of priority:
- Water
- X-Gal
- E. coli Biofilm
- Agar Gel
- Polyacrylamide
- Paper
Tom 10:56, 26 July 2007 (EDT) Just a note to take into account Osmolarity of the media, which I'm sure you've thought of already! Also, B-Galactosidase is an enzyme not a type of media?
Piping
How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?
This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.
Parts
List of parts from the registry we would like to test in-veso.
Rate of PoPs
Comparison with the efficiency of other chassis
Limitations of type of proteins made in the vesicles
Life span
Membrane Permeability
Physical Conditions
temperature, pH, media, centrifugation