IGEM:IMPERIAL/2007/Projects/In-Veso/Specifications

In-Veso Gene Expression: Specifications

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Characterization

Characterisation Chart

	PoPS Effect	Lifespan	Protocol	Membrane Traffic	Waste
Temperature
Media
pH
Piping
Centrifuge

Temperature

Range from 0-40^oC

Media

These are the different media the technique should be tested in, in order of priority:

1. Water
2. Agar Gel
3. β-Galactosidase
4. Polyacrylamide
5. Paper
6. E. coli Biofilm

Tom 10:56, 26 July 2007 (EDT) Just a note to take into account Osmolarity of the media, which I'm sure you've thought of already! Also, B-Galactosidase is an enzyme not a type of media?

pH

Range from 6.0 to 8.0

Piping

How narrow a pipe, and how fast, can the vesicles be passed through without bursting? Testing range..?

This is valuable for determining the spray system to be used in the Biofilm application. It also helps with laboratory manipulations - pipetting, etc.

Centrifuge

How fast can the vesicles be centrifuged without destruction? Testing range...?

Protocol

Above are two choices for making cell extract from E.coli. One of them is S30, and the other is the S12 method, which is a more rapid and cost-effective preparation. The cell extracts can also be obtained from a manufacturer.

This is a protocol for obtaining the cell extract S30

Physical Conditions

temperature, pH, media, centrifugation