IGEM:IMPERIAL/2007/Projects/chassis/Specifications: Difference between revisions
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*'''Initial Rate of Expression''' | *'''Initial Rate of Expression''' | ||
The rate of expression until steady state is reached. We can define the steady state and measure how long it takes to achieve this.<br> | The rate of expression until steady state is reached. We can define the steady state and measure how long it takes to achieve this.<br> | ||
*'''Rise Time''' | |||
The first time at which our system reaches the maximum level of expression | |||
*'''Peak Time''' | |||
The first time at which the rate of increse of expression is equal to zero. | |||
*'''Physical Requirements''' | *'''Physical Requirements''' | ||
Containment of the expression system e.g.test tube, and any media that is required. <br> | Containment of the expression system e.g.test tube, and any media that is required. <br> | ||
Revision as of 18:51, 7 August 2007
The characterisation of the parts and devices have been considered in terms of rate of gene expressing in the units Polymerase per Second (PoPS). However the characterisation of the chassis containing the expression systems has currently not been considered. If PoPS can be viewed as the rate of gene expression, the role of the chassis is that it contains a defined potential power of expression. So we can now define a relationship between the devices and expression system, if devices determine rate and expression system determines power. Analogy between the a power source e.g. battery and a device, battery defines total amount of available energy and device requirements determine the rate at which this is used.
Characteristics
- Life span of chassis
Measure of time that chassis is stable without expression, this is to give a measure of stability of stored expression systems.
- Life span of expression
Measure of the time that expression occurs for a given constructs until capacity reached, it is important to know when chassis will stop gene expression.
- Capacity
A measure of the total expression of a chassis for a given construct, can be seen as the total 'power' of expression a chassis can produce. Need to work out total synthesis taking into account the degradation
- Stability of Proteins
Degradation of a normalized protein i.e. Green Fluorescent Protein, important if our application depends on protein production e.g. a visual response from GFP.
- Initial Rate of Expression
The rate of expression until steady state is reached. We can define the steady state and measure how long it takes to achieve this.
- Rise Time
The first time at which our system reaches the maximum level of expression
- Peak Time
The first time at which the rate of increse of expression is equal to zero.
- Physical Requirements
Containment of the expression system e.g.test tube, and any media that is required.
- Cost
An indication of the cost of each expression system.
- Reproducibility
Measure of standard deviation of the measurements above. This will show how reliable the system to give a standard response.
