IGEM:IMPERIAL/2007/Wet Lab/Protocols: Difference between revisions
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Below is a databank of all the protocols that have been used in our projects. | Below is a databank of all the protocols that have been used in our projects. | ||
<br><br> | <br><br> | ||
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | |||
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | {| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | ||
|- style="background:#aabadd; color:Black" align="center" | |- style="background:#aabadd; color:Black" align="center" | ||
|| <font size= | |width="35%"|<font size=2>'''General Experiments'''</font>||width="65%"|<font size=2>'''Testing Experiments'''</font> | ||
|- style="background:#d5dcef; color:Black" valign="top" | |- style="background:#d5dcef; color:Black" valign="top" | ||
| | | | ||
<font size=3>''' | <font size=2><font color=green>'''Protein Purification'''</font> <br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * ''Purification of LuxR'' : [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * ''Purification of GFPmut3b'': | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]] | ||
<br> | |||
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | |||
* ''Optimisation of Fluorometer'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br> | |||
<font size=2><font color=green>'''Calibration Curve'''</font> <br> | |||
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]] | |||
* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]] | |||
<br> | |||
<font size=2><font color=green>'''Chassis Protocols'''</font> <br> | |||
* ''Preparation S30 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.1|Protocol]] | |||
* ''Preparation S12 Cell Extract '':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Protocol]] | |||
* ''Preparation of Reaction Mixture'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Protocol]] | |||
* ''Vesicles Formation'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/VF1.1|Protocol]] | |||
<br> | <br> | ||
<font size= | | | ||
* | <font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | ||
*''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]] | |||
<font size=2>'''Cell by Date'''</font> | |||
<br> | |||
<font color=green>'''Implementation'''</font> | |||
<br> | <br> | ||
* ''In Vivo Testing:'' [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.1|Results]] | |||
* ''Initial In vitro Testing'':[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.2|Results]] | |||
<font color=green>'''Testing/Validation<br>'''</font> | |||
*''Testing DNA Concentration in vitro'': Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/CBD4 |Results]] | |||
* ''Operating Temperature Range'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD2.2|Results]] | |||
*''Temperature Steps:'' Protocols and Results | |||
<font size=2>'''Infecter Detector'''</font> <br> | |||
<font color=green>'''Implementation<br>'''</font> | |||
*''In vivo Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.1|Results]] | |||
* ''Initial In Vitro Testing'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.2|Results]] | |||
<font color=green>'''Testing/Validation<br>'''</font> | |||
*''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]] | |||
* ''Testing of Purified LuxR'' :[[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]] | |||
* ''Characterization in vitro - AHL titration '': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]] | |||
|} | |||
<br clear="all"> | |||
OLD RESULTS + PROTOCOLS | |||
<font size=3>'''Fluorometer Testing'''</font> <br> | <font size=3>'''Fluorometer Testing'''</font> <br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]] | ||
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* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]] | ||
<br> | <br> | ||
<font size=3>'''Infector Detector'''</font> <br> | <font size=3>'''Infector Detector'''</font> <br> | ||
'''Phase 1'''<br> | '''Phase 1'''<br> | ||
'''Phase 2'''<br> | '''Phase 2'''<br> | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]] | ||
* [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]] | * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]] | ||
Latest revision as of 07:38, 25 October 2007
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ProtocolsBelow is a databank of all the protocols that have been used in our projects.
Fluorometer Testing
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