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Constructs Overview
Here are several tables listing the bricks which we are cloning, constructing and using, with their relevant details.
A new page is currently being developed!
Cloning Table
Status of biobricks cloned and their applications:
| I
| ID
| Part
| Description
| Status
| Ligation Target
| Use in Constructs
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| | 02 | BBa_I13522 | ptet-GFP | XL1, Maxi, Mini, BL21 | --- | Basic construct for chassis characterization
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| | 11 | BBa_E7104 | pT7 GFP | XL1, Maxi, Midi, Mini, BL21 | --- | CBD test construct (pT7)
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| | 30 | --- | pT7 (strong GFP) | XL1, Maxi, Mini, BL21 | --- | T7 test-construct
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| I | 18 | BBa_T9002 | ptet-luxR-plux-GFP | XL1, Maxi, Midi, BL21 | --- | Basic construct for chassis characterization
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| | 05 | BBa_R0040 | ptet | XL1, Midi, Mini | DsRedEx | CBD final (ptet), ID AHL sender
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| | | BBa_I719??? | pT7 | Ready | DsRedEx | CBD final construct (pT7)
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| | 07 | BBa_F2620 | ptet-luxR-plux | XL1, Midi, Mini | DsRedEx | ID final construct
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| | 21 | BBa_J23039 | ptet-luxI | XL1, Midi | --- | ID sender construct
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| | 24 | BBa_R0062 | plux | XL1, Midi | DsRedEx | ID construct 2
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| | 25 | BBa_J37032 | plux-GFP | XL1, Maxi, Midi | --- | ID construct 2
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| | 26 | BBa_S03119 | ptet-luxR | XL1, Midi | --- | ---
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| | 27 | pSB1A3 | Empty pSB1A3 | Problematic | --- | ---
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| | 13 | BBa_B0015 | Double terminator | XL1, Maxi, Midi, Mini | --- | General part construction
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| | 17 | BBa_I13507 | RFP | XL1, Midi | --- | Not being used anymore
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| | 19 | BBa_B0034 | strong RBS | XL1 | --- | General part construction
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| | 23 | BBa_I13504 | GFP | XL1, Maxi, Midi | pT7 | General part construction
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| | | BBa_I719??? | DsRed Express | Ordered | ptet, plux, pT7 | Reporter system for ID, Hrp
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| | | BBa_I719??? | HrpR-HrpS | Ordered | ??? | Hrp regulators
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| | | BBa_I719??? | pHrpL | Ordered | ??? | Hrp promoter
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| | | BBa_I719??? | HrpV | Ordered | ??? | Hrp inhibitor system
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Ligation Table
Status of parts ligated and their applications:
| I
| ID
| Part
| Description
| Status
| Parents
| Application
|
| | | BBa_I719??? | pT7 & GFP | Planned | pT7 + BBa_I13504 | CBD test construct (pT7)
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| | | BBa_I719??? | DsRedEx & pSB1A3 | Paused | DsRedEx + BBa_P1010 | Biobrick Submission
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| | | BBa_I719??? | pT7 & DsRedEx | Planned | pT7 + DsRedEx | CBD final construct (pT7)
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| | | BBa_I719??? | ptet & DsRedEx | Planned | BBa_R0040 + DsRedEx | CBD final construct (ptet)
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| | | BBa_I719??? | ptet-luxR-plux-DsRedEx | Planned | BBa_F2620 + DsRedEx | ID detector final construct 1
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| | | BBa_I719??? | plux-DsRedEx | Planned | BBa_R0062 + DsRedEx | ID detector final construct 2
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Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Maxi - maxiprepped; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3); status - stocks running low]
Parts from the Registry
Physical information of biobricks:
| A
| W
| G
| S
| ID
| Name
| Description
| Plasmid
| Resist
| Copy
| Size(B)
| Size(T)
| Location
| Conc.
(ng/ul)
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| A | W | G | | 02 | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | 500
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| A | W | G | | 05 | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | 60
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| A | W | G | | 07 | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | 80
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| A | X | G | | 11 | BBa_E7104 | T7 GFP | pSB1A2 | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | 100
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| A | W | G | | 13 | BBa_B0015 | Double terminator | pSB1AK3 | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | 100/70
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| A | | G | | 17 | BBa_I13507 | RFP | pSB1A2 | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | 80
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| A | W | G | | 18 | BBa_T9002 | GFP under lux promoter with LuxR | pSB1A3 | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | 460
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| A | W | | | 19 | BBa_B0034 | RBS | pSB1A2 | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | --
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| A | | G | | 21 | BBa_J23039 | Constitutive luxI | pSB1A2 | AmpR | High | 860bp | 2939bp | Well 11K, Plate 3 | 140
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| A | W | G | | 23 | BBa_I13504 | GFP | pSB1A2 | AmpR | High | 875bp | 2954bp | Well 16E, Plate 1 | 120/330
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| A | | G | | 24 | BBa_R0062 | lux promoter | pSB1A2 | AmpR | High | 55bp | 2134bp | Well 9G, Plate 1 | 90
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| A | | G | | 25 | BBa_J37032 | GFP under lux promoter | pSB1A2 | AmpR | High | 938bp | 3017bp | Well 8D, Plate 3 | 125/330
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| A | | G | | 26 | BBa_S03119 | constitutive LuxR | pSB1A2 | AmpR | High | 998bp | 3077bp | Well 19J, Plate 1 | 60
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| A | W | X | | 27 | pSB1A3 | pSB1A3 (blank) | pSB1A3 | Amp | High | 0bp | 2157bp | Well 23E, Plate 1 | --
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| | W | | | 30 | --- | T7 with GFP(strong) | --- | Kan? | ??? | --- | ??? | --- | 150
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Our Parts
Physical information of our ligated parts:
| A
| W
| G
| S
| ID
| Name
| Description
| Plasmid
| Resist
| Copy
| Size(B)
| Size(P)
| Stock
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| | | | | | BBa_I719022 | cI with GFP | pSB1A2 | AmpR | High | 933bp | 3988bp |
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| | | | | | BBa_I791021 | luxI coding region under ptet | pSB1A2 | AmpR | High | 860bp | 2939bp |
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| | | | | | BBa_I791??? | DsRed Express under ptet | pSB1A2 | AmpR | High | | |
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Legend: A (Available), W (Working) [W - working in vivo, W - working in vitro, X - faulty], G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Concentrations are given in ng/μl
Part Graveyard
General information on parts we do not like...
| A
| W
| ID
| Name
| Description
| Plasmid
| Size
| Status
| Notes
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| A | W | 01 | BBa_E0040 | GFP | pSB1A2 | 720bp | XL1, Mini | Redundant
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| A | | 03 | BBa_J5528 | pBad-GFP | pSB2K3 | 2093bp | failed-XL1 | ara construct abandoned
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| A | | 04 | BBa_J24677 | plux-GFP | pSB1A2 | 1787bp | XL1, Mini | Wrong GFP ligated
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| A | | 06 | BBa_I0500 | pBad | pSB2K3 | 1210bp | XL1, Mini, Midi | ara construct abandoned
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| A | W | 08 | BBa_F1610 | luxI | pSB1AK3 | 798bp | XL1, Mini, Midi | Substitute part J23039
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| A | W | 09 | BBa_R0010 | lacI-plac | pSB1A2 | 200bp | XL1, Mini | Lac construct abandoned
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| A | W | 10 | BBa_E1010 | RFP | pSB2K3 | 681bp | XL1, Mini | Substitute part I13507
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| A | | 12 | BBa_S03550 | araC | pSB1AC3 | 958bp | XL1, Mini, Midi | araC construct abandoned
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| | W | 14 | BBa_R0051 | pcI | pSB1A2 | 49bp | Biobrick | pcI construct abandoned
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| A | | 15 | BBa_J07037 | cI-GFP | pSB2K3 | 912bp | XL1, Mini | No RBS present on this part
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| A | W | 16 | BBa_E0240 | GFP | pSB1A2 | 876bp | XL1, Midi | Redundant
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| | W | 20 | --- | AraC | pSB1AC3 | 1095bp | Planned | araC construct abandoned
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| A | W | 28 | BBa_P1010 | pSB1A3 (ccd) | pSB1A3 | 0bp | failed-XL1 | Strain not available
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| A | W | 29 | BBa_P1010 | pSB1A2 (ccd) | pSB1A2 | 0bp | --- | Strain not available
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Construct Diagrams
The original (old) diagrams page was moved to old construct diagrams page. This page also contains the obsolete parts.
CBD Constructs
ptet GFP
pT7 GFP
ptet DsRed express
- Availability: Requires ligation
- Promoter can be found in registry as: BBa_R0040
- DsRed express is to be ordered from GeneArt
pT7 DsRed express
- Availability: Requires ligation
- Promoter is being PCRed from plasmid derived externally
- DsRed express is to be ordered from GeneArt
ID Constructs
pLux GFP with LuxR
 Ptet promoting LuxR, Plux promoting GFP
pLux GFP with LuxR
<<Two more constructs with DsRed express yet to be included>>
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