IGEM:IMPERIAL/2008/Prototype/Wetlab: Difference between revisions
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3.Transformation Protocol ''E.coli'' | 3.Transformation Protocol ''E.coli'' | ||
#[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]] | |||
4.[[/Protocols#Motility_Experiments | Motility Assays]] | 4.[[/Protocols#Motility_Experiments | Motility Assays]] |
Revision as of 02:49, 8 August 2008
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Team Strategy
We have decided to split the protocols work into 5 sections
- Cloning Strategy - Tom & Chris
- PCR - (Tom & Chris)
- B.subtilis - James
- Calibration curves
- Testing
Mircoscope work - Krupa
1, 2 and 3 should be completed by monday morning so as to start some lab work. Microscope information requested to be sent to Martin by Friday.
Testing Constructs
Click here to see the test constructs
Protocols
General Format
Link to the protocols we need!
1.Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for linear DNA in Bacillus subtilis
3.Transformation Protocol E.coli
5. PCR
These will collapse into the names on the official wiki...
Protocol 1
The actual protocol
Protocol 2
Second protocol
Shopping List
Reagents and Materials
Link to the Shopping list for Reagents and Materials.
Geneart
Click here to see the Construct ordered from Geneart
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