IGEM:IMPERIAL/2008/Prototype/Wetlab/parts

Adding Parts to the Registry

The registry has a simple guide about adding parts on the following link. Before we start to add our parts we should collect the following information about each of our parts:

• A part name
• The DNA sequence of the part you are making
• A short description of the part
• A long description of the part, including references

Most of this information is on the wiki under the [Sequence Page]. If the wet lab team could all contribute to adding these parts it would help speed things up. Once we have our parts we can then build up our constructs that we will be submitting to the registry.

Promoters

Promoter ctc

• Part name = promoter ctc
• Sequence =
• Promoter ctc is a sigma factor B dependent promoter found in B.subtilis. In B.subtilis endogenous sigma factor B is activated under mild stress from nutrient and physical stress response. The context with which we used the promoter ctc, was to take blue light as an input and give Polymerase Per Second(PoPS) as an output.

• Promoter ctc is a sigma factor B dependent promoter found in B.subtilis. In B.subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt or ethanol[1]. The context with which we used the promoter ctc, was to take blue light as an input and give Polymerase Per Second(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in B.subtilis [2].