IGEM:MIT/2005/Experiments: Difference between revisions
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J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter). | J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter). | ||
2. Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1) | 2. Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1) | ||
3. Input Experiments | 3. Input Experiments : Verify that flourescin oligo's enter cells. | ||
*<b>July wk 4:</b> | *<b>July wk 4:</b> | ||
Revision as of 07:41, 15 July 2005
Experimental Timeline
- July wk 3:
1. Receive primers [math]\displaystyle{ \rightarrow }[/math] Biobrick by PCR:
J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter).
2. Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1)
3. Input Experiments : Verify that flourescin oligo's enter cells.
- July wk 4:
FecA: Verify fecA promoter
Fuse scFv with FecA?
ToxR:
- Aug wk 1:
ToxR: -Receive synthesized J07007 (ctx promoter), J07009 (ToxR') -Begin Assembly of: -J07011 (ctx promoter :: gfp reporter) -J07024-26 (promoter::RBS::ToxR'::scFv 1-3) -J07027-28 (Positive and Negative controls, promoter::RBS::ToxR'::PhoA/MalE)
- Aug wk 2:
- Aug wk 3:
- Aug wk 4:
- Sept wk 1
Experiment Details
[[../Input reception Experiments/]]
[[../Receiver 1 experiments: ToxR/]]
[[../Receiver 2 experiments: FecA/]]
