IGEM:MIT/2005/Experiments

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Experimental Flowchart
Image:Check.gif indicates done!
Image:Pending.gif indicates pending...

Contents

Experiment Status

Stock-up MiniPreps
   Image:Check.gif grow up B0015, J13002, E0840
   Image:Check.gif overnight B0015, J13002, E0840
   Image:Check.gif miniprep B0015, J13002, E0840
   Image:Check.gif glycerol stock J13002


FecA (full length)
   Image:Check.gif PCR bb ends
   Image:Check.gif digest FecA + pSB1AK3-1 plasmid w/ E/S
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Check.gif transform
   Image:Check.gif overnights for miniprep
   Image:Check.gif identify correct clones
   Image:Check.gif overnights for freezer stock
   Image:Check.gif freezer stocks

Jessica

MalE
   Image:Check.gif PCR
   Image:Check.gif digest MalE + pSB1AK3-1 plasmid w/ E/S 
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Pending.gif transform
   Image:Pending.gif identify correct clones
PhoA
   Image:Check.gif PCR with higher annealing temperature
   Image:Check.gif digest PhoA+ pSB1AK3-1 plasmid w/ E/S 
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Pending.gif transform
   Image:Pending.gif identify correct clones

PhoA'
   Image:Pending.gif QuikChange

Annie

FecA promoter
   Image:Check.gif PCR with current primers with higher annealing temperature
   Image:Pending.gif PCR with newly designed primers
   Image:Pending.gif digest FecApro + pSB1AK3-1 plasmid w/ E/S 
   Image:Pending.gif gel purify each
   Image:Pending.gif ligate together
   Image:Pending.gif transform
   Image:Pending.gif identify correct clones (digest)
   Image:Pending.gif freezer stock
FecI
   Image:Check.gif PCR bb end
   Image:Check.gif digest FecI + pSB1AK3-1 plasmid w/ E/S
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Check.gif transform
   Image:Pending.gif identify correct clones (digest)
   Image:Pending.gif freezer stock
FecR
   Image:Check.gif PCR bb end
   Image:Check.gif digest FecR + pSB1AK3-1 plasmid w/ E/S
   Image:Check.gif gel purify each
   Image:Check.gif ligate together
   Image:Check.gif transform
   Image:Pending.gif identify correct clones (digest)
   Image:Pending.gif freezer stock
FecA'
   Image:Pending.gif QuikChange
FecR'
   Image:Pending.gif QuikChange

Project Experiments

  Input Device: fluorescein
  Head Receiver Unit: anti-fluorescein scFv
  Receiver 1: ToxR
  Receiver 2: FecA 
  Signal Processor: Exp. Here
  Actuator: Exp. Here

Experimental Timeline

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