IGEM:MIT/2005/Experiments
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[[../Experimental Flowchart/]]
indicates done! indicates pending...
Experiment Status
Promoter.RBS.scFv [[../JennyN Lab Notes#Jenny/]] See Detailed Notes Here. PCR start codon to scFv digest Promoter.RBS BB (recipient) digest scFvs (suffix donor) gel purify ligate promoter.RBS.svFv transform overnights for miniprep identify correct clones (digest), streak plates overnights for freezer stock freezer stocks promoter.RBS.scFv
Stock-up MiniPreps grow up B0015, J13002, E0840 overnight B0015, J13002, E0840 miniprep B0015, J13002, E0840 glycerol stock J13002
FecA (full length) PCR bb ends digest FecA + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones overnights for freezer stock freezer stocks
Jessica
MalE PCR digest MalE + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones
PhoA PCR with higher annealing temperature digest PhoA+ pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones PhoA' QuikChange
Annie
FecA promoter PCR with current primers with higher annealing temperature PCR with newly designed primers digest FecApro + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecI PCR bb end digest FecI + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecR PCR bb end digest FecR + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecA'
QuikChange
FecR'
QuikChange
Project Experiments
Input Device: [[../Input: Ligand#Experiments/|fluorescein]] Head Receiver Unit: [[../Receiver Head Unit: scFv#Experiments/|anti-fluorescein scFv]] Receiver 1: [[../Receiver 1: ToxR#Experiments/|ToxR]] Receiver 2: [[../Sensor 2#Experiments/|FecA]] Signal Processor: [[../Signal processing#Experiments/|Exp. Here]] Actuator: [[../Actuator#Experiments/|Exp. Here]]
[[../Experimental Timeline/]]