IGEM:MIT/2005/Experiments
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[[../Experimental Flowchart/]]
indicates done! indicates pending...
Experiment Status
Stock-up MiniPreps grow up B0015, J13002, E0840 overnight B0015, J13002, E0840 miniprep B0015, J13002, E0840 glycerol stock J13002
FecA (full length) PCR bb ends digest FecA + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform overnights for miniprep identify correct clones overnights for freezer stock freezer stocks
Jessica
MalE PCR digest MalE + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones
PhoA PCR with higher annealing temperature digest PhoA+ pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones PhoA' QuikChange
Annie
FecA promoter PCR with current primers with higher annealing temperature PCR with newly designed primers digest FecApro + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecI PCR bb end digest FecI + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecR PCR bb end digest FecR + pSB1AK3-1 plasmid w/ E/S gel purify each ligate together transform identify correct clones (digest) freezer stock
FecA'
QuikChange
FecR'
QuikChange
Project Experiments
Input Device: [[../Input: Ligand#Experiments/|fluorescein]] Head Receiver Unit: [[../Receiver Head Unit: scFv#Experiments/|anti-fluorescein scFv]] Receiver 1: [[../Receiver 1: ToxR#Experiments/|ToxR]] Receiver 2: [[../Sensor 2#Experiments/|FecA]] Signal Processor: [[../Signal processing#Experiments/|Exp. Here]] Actuator: [[../Actuator#Experiments/|Exp. Here]]
[[../Experimental Timeline/]]