IGEM:MIT/2005/Receiver 1: ToxR: Difference between revisions
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#All parts specified | #All parts specified | ||
## and maybe ready for synthesis ... | ## and maybe ready for synthesis ... | ||
fwd: 5' GAATTC GCGGCCGC T TCTAGA ATGAAACAGAGCACCATTG 3' | |||
EcoRI NotI XbaI Primer | |||
rev: 5' CTGCAG GCGGCCGC T ACTAGT TTATTA TTATTTCAGGCCCAGCGCC 3' | |||
PstI NotI SpeI Stop Primer | |||
==Open Issues/Questions== | ==Open Issues/Questions== |
Revision as of 09:31, 13 July 2005
Receiver Device #1 -- ToxR
POC
wiki-Will
Function
To dimerize in the presence of the input signal and cause gene expression to turn on.
Device Depiction
Information path ...
- Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
- Fluoroscein binds to adjacent ToxR'-scFv fusions.
- Causes ToxR' dimerization
- GFP expressed @ CTX_promoter
- As a function of ToxR dimerization
People path ...
- Maxine gives us Fluoroscein, or identifies something else
- Jenny's scFv binds to Maxine's antigen
- Then, Jenny's scFv induces ToxR' to dimerize
- The dimerization leads to the expression of Jessica's output @ CTXpromoter [C1, maybe]
- Ray makes the whole system look like "we meant to do that."
Device Parts
ToxR'-scFv, CTX-GFP, ToxR'-malE, Toxr'-PhoA, oligo-flour
Current Status
- Going from DNA sequence information to physical genetic material that works inside living cells.
- see device parts for what we need ...
- Getting Signal Into Periplasm
- CAN WE GET OLIGO's AND RUN TESTS NOW ???
- All parts specified
- and maybe ready for synthesis ...
fwd: 5' GAATTC GCGGCCGC T TCTAGA ATGAAACAGAGCACCATTG 3' EcoRI NotI XbaI Primer rev: 5' CTGCAG GCGGCCGC T ACTAGT TTATTA TTATTTCAGGCCCAGCGCC 3' PstI NotI SpeI Stop Primer
Open Issues/Questions
- !
- Can we get the signal in the periplasm?
- Or, can we adapt or extend the ToxR receiver device so that it accept a signal at the outer membrane.
- ToxR/scFv Fusion - Can we know with any more certainty that the fusion will work?